Journal: Cell reports

Article Title: Pleiotrophin regulates presynaptic assembly and function through heparan sulfate-dependent binding to neurexin1

doi: 10.1016/j.celrep.2026.117827

Figure Lengend Snippet:

Article Snippet: Anti-Synapsin1 , Synaptic Systems , Cat# 106011; RRID:AB_2619772.

Techniques: Virus, Recombinant, Magnetic Beads, Mass Spectrometry, shRNA, Sequencing, Software

Journal: bioRxiv

Article Title: Impaired astrocyte-to-neuron cholesterol trafficking drives synaptic dysfunction in Rett syndrome

doi: 10.64898/2026.07.29.741426

Figure Lengend Snippet: ( A ) Representative images of primary branches from WT neurons treated for 24 hours with WT-ACM or KO-ACM, supplemented or not with cholesterol (0.1 μg/ml), and immunostained for Synapsin1/2 (Syn1/2; green), Shank2 (red) and Map2 (white). Scale bar=5 µm. ( B-D ) Violin plots indicate the median (dashed line) and 25 th and 75 th percentiles (dotted lines) of Synapsin1/2 (B), Shank2 (C) and colocalized puncta number (D). Values for puncta number are expressed as percentages with respect to neurons treated with WT-ACM. n=40/50. Statistical analysis was performed by 2-way Anova, followed by Sidak post-hoc test; *p<0.05, ***p<0.001.

Article Snippet: For the analyses of synaptic puncta density, neurons were stained with antibodies against Synapsin1/2 (#106006, Synaptic System; 1:500), Shank2 (#162 211, Synaptic System; 1:300) and Map2 (#8707, Cell Signalling; 1:1000).

Techniques:

Journal: bioRxiv

Article Title: Impaired astrocyte-to-neuron cholesterol trafficking drives synaptic dysfunction in Rett syndrome

doi: 10.64898/2026.07.29.741426

Figure Lengend Snippet: ( A ) Representative images of primary branches from WT and Mecp2 HET neurons, treated for 24 hours with cholesterol or with vehicle (0.1 μg/ml), and immunostained for Synapsin1/2 (Syn1/2; green), Shank2 (red) and Map2 (white). Scale bar=5 µm. ( B-D ) Violin plots indicate the median (dashed line) and 25 th and 75 th percentiles (dotted lines) of Synapsin1/2 (B), Shank2 (C) and colocalized puncta number (D). Values for puncta number are expressed as percentages with respect to untreated WT neurons. n=57/69. Statistical analysis was performed by 2-way Anova, followed by Sidak post-hoc test; **p<0.01, ***p<0.001. ( E ) Representative images of axon initial segment (AIS) stained with anti-AnkyrinG (AnkG; red). Scale bar=20 µm. ( F,G ) Violin plots indicate the median (dashed line) and 25 th and 75 th percentiles (dotted lines) of AIS length in WT and Mecp2 HET neurons (at DIV14) after cholesterol supplementation or treated with vehicle (F). The frequency distribution of values of AIS length is shown in (G). n=70/exp.groups. ( H ) Violin plots indicate the median (dashed line) and 25 th and 75 th percentiles (dotted lines) of the distance of AIS from the soma in WT and Mecp2 HET neurons (at DIV14) after cholesterol supplementation or treated with vehicle. n=70/exp.groups. *p<0.05, by 2-way Anova, followed by Sidak post-hoc test;

Article Snippet: For the analyses of synaptic puncta density, neurons were stained with antibodies against Synapsin1/2 (#106006, Synaptic System; 1:500), Shank2 (#162 211, Synaptic System; 1:300) and Map2 (#8707, Cell Signalling; 1:1000).

Techniques: Staining

Journal: Nature Communications

Article Title: Brain FGF2 and NCAM1 contribute to FGFR1-dependent progression of estrogen receptor-positive breast cancer brain metastases

doi: 10.1038/s41467-026-73726-5

Figure Lengend Snippet: a RNA-sequencing schematic for UCD12 and UCD65 cells in serum-free media treated with vehicle, 10 ng/mL FGF2, or 20ug/mL NCAM1 for 24 hr ( n = 3). b Venn diagram shows unique and shared significant gene signatures following FGF2 and NCAM1 treatment. c Pie chart of gene signature categories differentially induced by NCAM1 treatment. d log(FDR) of significant neural gene signatures differentially enriched or repressed following NCAM1 treatment. e Immunofluorescent staining of PSD95 and Synapsin1/2 in shNC and shFGFR1 UCD65 spheroids in co-culture with organotypic brain slices from aged NSG female mice ( > 59w) for 3 days. Punctate colocalization ( n = 4 independently stained organotypic co-cultures per condition) was quantified. 5000 GFP+ shNC and shFGFR1 UCD12 ( f ) or UCD65 ( g shNC vs shFGFR1 #1 p = 9.61E-07) cells were plated on coverslips with 315,000 primary hippocampal neurons (DIV: 8) and imaged every 12 hours for 6–9 days via Incucyte. Cell migration was measured and mean distance per cell within each biological replicate ( n = 4 UCD12 coverslips per condition, n = 3 UCD65 coverslips per condition) was plotted. Right: representative images of UCD65 cells. Scale bars: 20 µm. h 5000 UCD12 and UCD65 cells were plated on a monolayer of shEV or shNCAM1 SY5Y cells and imaged every 12 hours for 6.5 days via Incucyte. Cell migration over time was measured and mean distance per cell within each biological replicate ( n = 5 per condition) was plotted. For e , g , h data were analyzed by 1-way ANOVA followed by Fisher’s LSD test. f was analyzed with two-tailed t-test. Source data are provided as a Source Data file. a Created in BioRender. Cittelly, D. ( https://BioRender.com/0zvir15 ).

Article Snippet: Primary antibodies against GFAP (Invitrogen 12-0300, 1:200), FGF2 (Novusbio NBP3-15367, 1:100), pFGFR1 (Y654, Abcam ab59194, 1:400), NCAM1 (R&D AF2408, 1:200), PanCK (DAKO M0821, 1:100), Synapsin1/2 (Synaptic Systems 106004, 1:1000), PSD95 (Invitrogen MA1-045, 1:500), and pERK (Cell Signaling 9101S, 1:200) were incubated for 2 hours or overnight at RT.

Techniques: RNA Sequencing, Staining, Co-Culture Assay, Migration, Two Tailed Test